2024 Stat5 reporter assay

Stat5 reporter assay

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August undefined, 2024

WebA highly sensitive method for determination of insulin receptor (IR) kinase activity in whole cells, which is based on a STAT5 (signal transducer and activator of transcription 5)-dependent reporter gene assay, has been developed. WebTo analyze STAT5A phosphorylation: based on the following gating strategy (1) forward scatter (FSC) vs. side scatter (SSC) and (2) CD3 vs. SSC, the T cells (CD3 +) were separated ( Supplementary Figure 1 ). Now, after clear …

STAT5 Reporter (Luc) – Ba/F3 Cell line - BPS Bioscience

WebThe following assays are designed for 96-well format. To perform the assay in a different format, the cell number and reagent volume should be scaled appropriately. A. Dose response of STAT5 reporter (Luc) -U937 cells to human GM-CSF 1. Harvest STAT5 reporter (Luc)-U937 cells from culture in the Growth Medium 8A and seed cells at a WebMay 1, 2011 · One day after transfection, β-galactosidase reporter assays were performed. Specific activities from cells transfected with p ... Stat5 reporter gene expression in tissue sections from liver, kidney, spleen, thymus, and uterus. Indicated organs were isolated from C57BL/6 wild-type mice (row 1) and Stat5-REIN reporter mice (rows 2 and 3 ... physical therapy in hawthorne

Cytokine Signaling Pathway Analysis Response Element Firefly …

WebOct 23, 2024 · Reporter assays demonstrated that Shd1 repressed Stat5-mediated transcription in a dose-dependent manner without affecting Stat5 stability or phosphorylation. Nakajima et al. (2009) concluded that SHD1 is a cytokine-inducible negative-feedback regulator of STAT5. WebLike the Kinase receptor activation (KIRA) assay, developed by Sadick et al. [1], the STAT5 phosphorylation assay was performed using two separate microtiter plates: the first one … physical therapy in hazleton pa

Cellular LanthaScreen and beta-lactamase reporter assays for …

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WebJan 30, 2024 · We describe here the development and validation of a reporter gene assay (RGA), based on an IL-5-dependent TF-1 cell line variant we established that stably … WebFeb 24, 2024 · General strategy for the design of the reporter cell line: [a] signal through most of the immunologically relevant receptors results in the activation of NF-κB 23, 30, … physical therapy in henning mnWebReadily assessable STAT5-inducible SEAP reporter activity The stability for 20 passages has been verified Functionally tested and guaranteed mycoplasma-free Applications of HEK-Blue™ IL-9 cells: Detection of human IL-9 (100 pg/ml - 100 ng/ml) and murine IL-9 (30 pg/ml -100 ng/ml) Screening of anti-IL-9 and anti-IL-9R antibodies References: 1. physical therapy in hays ks

"WebReporter Vector primer 4 (RVprimer4) binding region 4171–4190 Sequence information for the pGL4 Vectors is available online at: www.promega.com/vectors/ Example Protocol In … " - Stat5 reporter assay

Stat5 reporter assay

IL-5 reporter cells IL-5 cell-based assay InvivoGen

WebAug 3, 2016 · STAT5 deficiency prevented IL-3-mediated inhibition of osteoclastogenesis, suggesting a key role of STAT5 in IL-3-mediated inhibition of osteoclast differentiation. In … WebFeb 2, 2024 · STAT5 is a key member of the JAK/STAT core cancer pathway, activated by a plethora of cytokines and growth factors to regulate a wide spectrum of physiologic …

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WebSTAT Reporter Cell Line - Tool for Screening of Drugs Targeting JAK/STAT Pathway; Imaging with CRISPR/Cas9; CoV-S Pseudotyped Lentivirus - Efficient Tool for Anti-CoV … WebIn an attempt to use the cells for large-scale chemical screens to identify JAK3 inhibitors, we established a cell line, 32D/IL-2Rβ/6xSTAT5, stably expressing a STAT5 reporter gene. Treatment of this cell line with IL-2 or IL-3 dramatically increased the reporter activity in a high-throughput format.

WebMoreover, bioinformatics analyses and luciferase reporter assay indicated that exosomal miR-182-5p was bound to PPARG 3' UTR sequence and inhibited the expression of PPARG. Collectively, our findings provided a new role of EPCs-EXO in the clinical treatment of diabetic skin wounds. ... (STAT5) to control EPC cell-cycle progression 44. In ... WebJul 15, 2002 · The STAT5 reporter plasmid containing a minimal promoter and 3 repetitive STAT5 binding sites from the β-casein promoter (pSP72-MP-STAT5-luciferase) ... STAT5 transactivation assay of AS-E2 cells that had been cotransfected with 4 μg wild-type CBP, CBP Leu607Phe, adenoviral E1A, or mutant E1A proteins E1AΔCR1 and E1AΔCR2. ...

WebThis assay utilized the growth factor-dependent human erythroleukemia TF1 cell line engineered to express a green fluorescent protein-STAT5 fusion protein. Furthermore, a complementary beta-lactamase reporter gene assay was developed to analyze the transcriptional activity of STAT5 downstream of JAK2 in TF1 cells. WebReadily assessable STAT5-inducible SEAP reporter activity The stability for 20 passages has been verified Functionally tested and guaranteed mycoplasma-free Applications of HEK …

WebHygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line Choose a response element ISRE SBE SIE STAT5 RE Size 20µg Catalog number selected: E4041 $ 435.00 Your price: Log in Add to Cart Add to Helix Cytokine Signaling Pathway Analysis Response Element Firefly Luciferase Vectors …

WebApr 1, 2011 · The STAT5 reporter assays in a 96-well plate format. The 32D/IL-2Rβ/6xSTAT5 reporter cells were deprived of WEHI-3 cell-conditioned medium for 6 h. The cells were then resuspended in the absence of WEHI-3 cell-conditioned medium (4 × 10 5 cells/mL) and treated with IL-2 (20 ng/mL) or IL-3 (1 ng/mL) to activate JAK3 or JAK2, respectively. physical therapy in hereford mdWebJan 27, 2003 · As Dex was used in the STAT5 reporter assay to enhance response , we examined whether the effect of E 2 depended on the presence of glucocorticoid. GH-induced STAT5 activity was significantly lower in the absence than the presence of 250 nM Dex (9.1 ± 0.6-fold and 19.6 ± 1.0-fold, respectively; P 2 without or with Dex (55 ± 2% and 58 ± 1% ... physical therapy in hereford txWebFeb 2, 2024 · Two duplicate plates were set up, one for SEAP reporter assay and one for CellTiter-Glo cell viability assay. SEAP was determined using QUANTI-Blue (InvivoGen, rep-qbs). physical therapy in hermitage tnWebMar 19, 2013 · Reporter assay to monitor oncogenic FLT3-ITD activity. As FLT3-ITD strongly activates STAT5, 3, 4, 12, 13, 20, 24, 26 we employed a FLT3-ITD-driven STAT5 activation reporter assay for the screen ... physical therapy in hendersonville tnWeb6.2 STAT5 Signaling. STAT5 was shown to be a mediator of erythropoietin-induced neuroprotection in a rat model of global cerebral ischemia (Zhang, Wang, Cao, Gao, & … physical therapy in herndon vaWebAssay Interferences Interference testing should be relevant to the patient population that will be tested with a given assay endogenous substances - highest reported clinically relevant … physical therapy in henderson tnWebThe STAT5 Reporter (Luc)-Ba/F3 cell line is designed for monitoring STAT5 signal transduction pathways. It contains a firefly luciferase gene driven by the STAT5 response … The One-Step™ Luciferase Assay System is designed for high-throughput, sensitive … Recombinant Sortase A, His-tag (BPS Bioscience #71086) dose-dependent … physical therapy in hickory nc