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Pmon530

WebJun 1, 2024 · The resultant pMON530-TCD11-GFP plasmids were introduced into tobacco (Nicotiana tabacum) leaves and co-cultured at 25 °C for 48 h. At the same time, the pMON530-GFP empty carrier was used as a control. WebpMON530-GFP, E. coli MM294 carrying a helper plasmid pRK2013, and A. tumefaciens GV3111 containing the Ti-plasmid pTiB6S3SE. Recombinant A. tumefaciens GV3111

Transcription Factor OpWRKY3 Is Involved in the Development …

WebAgrobacterium-mediated transformation is the preferred method to transform plants because it usually results in simple insertions of target DNA. In this article, we describe a method for Agrobacterium-mediated transformation of the inbred B. distachyon lines Bd21 and Bd21-3. Embryogenic callus induced from immature embryos is co-cultivated with ... Webfragment from the plasmid pMON530 in Escherichia coli strain NM294 was used as the probe for transformed tissue DNA. Plas-mid DNA was isolated from E. coli using an alkaline lysis method (Liszewski et al., 1989; Sambrook et al., 1989). PCR amplification of the nos fragment for probe preparation or for plant DNA analysis was carried out ac- lakha bhajan https://adl-uk.com

The rice ALS3 encoding a novel pentatricopeptide repeat protein is ...

WebApr 27, 2024 · Then, the constructed vector and the pMON530 (used as the control) was transformed into the ASE strain and expressed in tobacco leaves, respectively. In the leaves of control vector transformed plant, the fluorescence of GFP was detected in the cytoplasm and nucleus (Figure Figure3 3). In contrast (to this), the fluorescence of SmWRKY1/GFP ... WebApr 27, 2024 · To experimentally confirm the subcellular localization of SmWRKY1, SmWRKY1 was cloned into the pMON530 vector to fuse with green fluorescent protein (GFP) reporter gene to generate vector pMON530-SmWRKY1-GFP. WebNov 8, 1996 · Calli were transformed with pPCVICEn4HPT ( A and B ), pC1S1Ti ( C and D ), pMON530 ( E and F ), or p35ScCKI1 ( G and H) and cultured for 18 days in the presence [ (A), (C), (E), and (G)] or absence [ (B), (D), (F), and (H)] of cytokinin [ trans … lakha bhajan ringtone download

Cadmium and iron transport by members of a plant metal …

Category:[15] Improved vectors for plant transformation ... - ScienceDirect

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Pmon530

Altered expression of Arabidopsis genes in response to a ...

WebApr 28, 2015 · The final construct 35S:EYFP-LeNRT2.3/pMON530 and the empty vector 35S:EGFP/pMON530 were transiently expressed in onion epidermal cells using a particle gun-mediated system (PDS-1000/He; Bio-Rad). The bombarded cells were held in the dark at 22 °C for 12 h followed by YFP imaging using confocal microscopy (Carl Zeiss; LSM … WebJan 1, 2024 · The full-length ORF of SmMYB1 (without the stop codon) was fused to a green fluorescent protein (GFP) gene under the control of the CaMV 35S promoter within a pMON530 vector to generate a SmMYB...

Pmon530

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WebMar 1, 2024 · This was then ligated into the pMON530-GFP vector, in frame with green fluorescent protein (GFP) under the control of the CaMV 35S promoter. The resultant pMON530-TCM1-GFP plasmids were introduced into tobacco (Nicotiana tabacum L.) leaves and cocultured at 25°C for 2 d. In the meantime, pMON530-GFP empty vector was used … WebNov 18, 2014 · As a control, vector DNA containing the CaMV 35S promoter alone (pMON530) was introduced into Agrobacterium. Agrobacterium infusion assays and RNA isolation. Vacuum infiltration of Arabidopsis thaliana plants with Agrobacterium cultures was performed essentially as described .

WebJan 14, 2010 · The resulting plasmid pMON530-CLS was first introduced into Agrobacterium tumefaciens by electroporation and then transformed into the Arabidopsis las mutant by floral dip method (Clough and Bent 1998). Transgenic plants were selected on MS medium containing 20 mg/L Kanamycin. DNA Isolation and Gel Blot Analysis Web7.根据权利要求6所述一种采用草莓hy5基因调控草莓果实成熟周期的方法，其特征在于，所述过表达载体为pmon530-hy5。8.根据权利要求6所述一种采用草莓hy5基因调控草莓果实成熟周期的方法，其特征在于，所述宿主菌为农杆菌gv3101感受态。

WebThe constructed expression vector pMON530-SmERF1L1-GFP. The AP2/ERF transcription factor SmERF1L1 regulates the biosynthesis of tanshinones and phenolic acids in Salvia miltiorrhiza

Webrived from the binary vector pMON530 [15]. In designing the T-DNA of the binary vector the primary aim was to provide an organisational structure with a IacZ' region immediately 3' of the right T-DNA border and 'overdrive' enhancer …

WebDownload scientific diagram Schematic representation of the T-DNA region of the binary vectors pMON337-TGMV-GFP and pMON530-GFP. jeni\\u0027s chicagoWebNational Center for Biotechnology Information jeni\u0027s froseWebApr 13, 2000 · After cloning this product into the TA vector (Invitrogen), a BglII/KpnI fragment containing the SHP2 cDNA was cloned into the plant transformation vector pMON530 (Monsanto). lakha bhajan mp3 song downloadWebMar 21, 2016 · The resultant pMON530-TCM5-GFP plasmids were introduced into tobacco (Nicotiana tabacum) leaves and co-cultured at 25 °C for 2 days. At the same time, the pMON530-GFP empty carrier was used as a control. Then the GFP fluorescences in tobacco cells were observed using a Zeiss confocal laser scanning microscope ... lakha bhajan mata raniWebDot blot assay of transgenic (Tr1,Tr2,Tr3) tomato plants and plasmid pMON530 (P). Membrane probed with Alk Phos labelled CKI-1 gene PCR fragment Source publication Differential response of tomato... lakha bhajan mp3WebApr 9, 2015 · The pMON530:CaMV35S:ASL3-GFP plasmid was introduced into tobacco cells using Agrobacterium-mediated infection method. Meanwhile, empty GFP vector was used as a control. As a result, the green fluorescent signals of ASL3-GFP fusion protein perfectly overlapped with chloroplast autofluorescence in transformed tobacco mesophyll … jeni\u0027s brambleberry ice creamWebAug 25, 2016 · a pMON530-GFP, used as the blank vector control; b pMON530-SmDXS1-GFP; c pMON530-SmDXS2-GFP. Full size image. PCR and qRT-PCR analysis of transgenic hairy roots. The observed differential expression of the two SmDXS genes suggested that they perform distinct regulatory roles in the first step of the MEP pathway. jeni\\u0027s everything bagel