Fhcc98
WebHepG2, Bel-7402, FHCC98, and Huh-7—were cul-tured under standard cell culture conditions in Dul-becco’s modified Eagle’s medium (DMEM) (GIBCO, Life Technologies) supplemented with 10% fetal bo-vine serum (GIBCO), 1% L-glutamine, 1% penicillin-streptomycin, and 1% nonessential amino acids in a 5% CO 2-humidified chamber. … WebDownload scientific diagram Expression of C1orf61 in the HCC cell lines. A) C1orf61 expression levels of the indicated cell lines were assessed by Western blotting. C1orf61 …
Fhcc98
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WebJun 11, 2024 · Human HepG2, FHCC98, and HEK293T cells were obtained from the China Center for Type Culture Collection (Wuhan, China) and were cultured as previously … WebOur previous study demonstrated that overexpression of HAb18G/CD147 promotes invasion by interacting with integrin α3β1. However, it has never been investigated whether α3β1 is solely responsible for this process or if other integrin family members also interact with HAb18G/ CD147 in human hepatoma cells.
WebSep 1, 2024 · The human liver cancer cell lines (FHCC98, Bel7404), kidney cancer cell line A498, stomach cancer cell lines (HGC, BGC), and human non-small cell lung cancer cell lines (A549, H460, H522) were cultured in RPMI-1640 in the presence of 10% fetal bovine serum (Gibco, Life Technologies). The cervix cancer cell HeLa, liver cancer cell line … WebPrecipitates from irrelevant antibody (Mouse IgG) were used as a negative control. (B) Expression of HAb18G/CD147 in FHCC98 cell lysates by Western blot. (C) Expression …
WebDec 14, 2014 · Brachyury overexpression has been reported in various human malignant neoplasms, but its expression and function in hepatocellular carcinoma progression and … WebMethods: Human SMMC-7721 and FHCC98 cells were cultured and transfected with siRNA fragments against HAb18G/CD147. The expression levels of HAb18G/CD147 and integrin α6β1 were determined by immunofluorescent double-staining and confocal imaging analysis. Co-immunoprecipitation and
WebDec 14, 2014 · Methods: The expression of Brachyury in human HCC (SMMC7721, HepG2, FHCC98, and Hep3B) and control cell lines was analyzed using quantitative reverse …
WebJul 1, 2024 · The following human liver cancer cell lines were used: (a) SMCC7721 and Hep3B cells were kindly provided by Dr. Zhiyong Mao (Tongji University), (b) HCCLM9 … pantalone sci kappaWebSep 23, 2009 · Human SMMC-7721 and FHCC98 cells were cultured and transfected with siRNA fragments against HAb18G/CD147. The expression levels of HAb18G/CD147 and … pantalone sci donnaWebJul 13, 2024 · To investigate the role of miR-148a on tumor growth, an orthotopic liver cancer model was used. As shown in Supplementary Figure 2b and c, the tumors formed by miR-148a-3p sponge-expressing FHCC98 cells were bigger than those formed by control FHCC98 cells. In contrast, tumors formed by Pri-miR-148a expressing HepG2 cells were … pantalone sci donna colmarWebJun 1, 2013 · c‐Myc (Myc) plays an important role in normal liver development and tumorigenesis. We show here that Myc is pathologically activated in and essential for promoting human hepatocellular carcinoma (HCC). Myc induces HCC through a novel, microRNA (miRNA)‐mediated feedback loop comprised of miR‐148a‐5p, miR‐363‐3p, … pantalone sci cmpWebDec 17, 2014 · Western blot analysis indicated that the tetrandrine and chloroquine combination activated the apoptotic-related proteins PARP and caspases in cancer cells including A549, Huh7, Hela, U251, Calu1 and FHCC98 (Figure 2B and Supporting Information Fig. S1). To further examine the role of caspases in the combination-induced … pantalones de caza berettaWebDec 17, 2014 · Combination of tetrandrine and chloroquineinduced induce cell apoptosis CONCLUSIONS & IMPLICATIONS: Our findings suggest that the combination of tetrandrine and chloroquine has synergistic... pantalone sci uomoWebJul 13, 2024 · Here miR-148a knockout (KO) mice were used to investigate the intrinsic role of miR-148a in liver physiology and hepatocarcinogenesis in mice. miR-148a downregulation was found to be correlated... pantalone scozzese